The Second Affiliated Hospital of Wenzhou Medical University 2 articles published in JoVE Neuroscience Targeted Knockdown of Genes in the Choroid Plexus Yuwen Yang*1, Chenxing Qi*1, Lanxin Hu1, Cheng Zheng1, Xuhang Li1, Wu Zheng1, Yiyun Weng2, Haiyan Lin3,4 1State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, 2Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, 3Rehabilitation Medicine Center, The Second Affiliated Hospital of Wenzhou Medical University, 4Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation, Wenzhou Medical University Here, we describe a method to selectively alter gene expressions in the choroid plexus while avoiding any impact in other brain areas. Developmental Biology In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity Shen Zhang1, Yonggen Wu1, Yimin Weng2, Zhihui Xu1, Wenmin Chen3, Dahan Zheng4, Wei Lin5, Jun Liu6, Ying Zhou1,7 1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
