3 articles published in JoVE
Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin Kun Lv1,2, Song Gao1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, 2Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University We present a workflow for segmenting and quantifying trabecular bones for 2D and 3D images based on the bone's outer boundary using an ImageJ plugin. This approach is more efficient and accurate than the current manual hand-contouring approach, and provides layer-by-layer quantifications, which are not available in current commercial software.
Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM) Jing Men1,2, Jason Jerwick2,3, Penghe Wu1,2, Mingming Chen3,4, Aneesh Alex2,3, Yutao Ma4, Rudolph E. Tanzi5, Airong Li5, Chao Zhou1,2,3 1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.
Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology Xiaofeng Cui*1,2,3, Guifang Gao*2,4, Tomo Yonezawa5,6, Guohao Dai1 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Stemorgan Inc., 3Institute of Advanced Study, Technical University of Munich, 4Institute of Virology, School of Medicine, Wuhan University, 5Department of Molecular and Experimental Medicine, The Scripps Research Institute, 6Research Institute for Biomedical Sciences, Tokyo University of Science The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.