4 articles published in JoVE
Establishment of a Mouse Severe Acute Pancreatitis Model using Retrograde Injection of Sodium Taurocholate into the Biliopancreatic Duct Xiaochong Zhou1, Haifeng Chen1, Xing Wei1, Yang He2,4,5, Chunfang Xu1, Zhen Weng2,3,4,6 1Department of Gastroenterology, the First Affiliated Hospital of Soochow University, 2MOE Engineering Center of Hematological Disease, Soochow University, 3Cyrus Tang Hematology Center, Soochow University, 4National Clinical Research Center for Hematologic Diseases, the First Affiliated Hospital of Soochow University, 5MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 6Collaborative Innovation Center of Hematology, Soochow University A mouse model of severe acute pancreatitis is described herein. The procedure presented here is very rapid, simple, and accessible, thereby potentially allowing the study of the molecular mechanisms and different therapeutic interventions in acute pancreatitis in a convenient way.
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts Yujie Huang*1, Yizhou Tao*1, Chang Cai1, Jin Chen1, Cheng Ji1, Stanley Aniagu2, Yan Jiang1, Tao Chen1 1School of Public Health, Soochow University, 2Toxicology, Risk Assessment and Research Division, Texas Commission on Environmental Quality This protocol uses an immunofluorescence assay to detect PM2.5-induced DNA damage in the dissected hearts of zebrafish embryos.
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability Ban-Ruo Li*1,2, Jia Wu*1,2, Hua-Shan Li1,2, Zhi-Hui Jiang1,2, Xiu-Min Zhou1,2, Cai-Hua Xu1,2, Ning Ding1,2, Juan-Min Zha1,2, Wei-Qi He1,2 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes Adam C. Vandergriff1,2, Michael Taylor Hensley1,2, Ke Cheng1,2,3 1Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 2Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, 3The Cyrus Tang Hematology Center, Soochow University The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.