2 articles published in JoVE
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability Ban-Ruo Li*1,2, Jia Wu*1,2, Hua-Shan Li1,2, Zhi-Hui Jiang1,2, Xiu-Min Zhou1,2, Cai-Hua Xu1,2, Ning Ding1,2, Juan-Min Zha1,2, Wei-Qi He1,2 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes Adam C. Vandergriff1,2, Michael Taylor Hensley1,2, Ke Cheng1,2,3 1Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 2Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, 3The Cyrus Tang Hematology Center, Soochow University The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.