3 articles published in JoVE
Quantification of Proliferative and Dead Cells in Enteroids Hua-Shan Li*1, Shao-Fang Xu*1, Jian-Ying Sheng*1, Zhi-Hui Jiang1, Jing Wang1, Ning Ding1, Tao Wang1, Matthew A. Odenwald2, Jerrold R. Turner2,3, Wei-Qi He1, Hong Xu1, Juan-Min Zha1 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3 The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability Ban-Ruo Li*1,2, Jia Wu*1,2, Hua-Shan Li1,2, Zhi-Hui Jiang1,2, Xiu-Min Zhou1,2, Cai-Hua Xu1,2, Ning Ding1,2, Juan-Min Zha1,2, Wei-Qi He1,2 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots Lu Xu1, Qiwen Shen1, Zhongqi Mao2, L. James Lee3, Ouliana Ziouzenkova1 1Department of Human Sciences, The Ohio State University, 2Department of Minimally Invasive Surgery, The First Affiliated Hospital of Soochow University, 3NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University Here, we present a protocol for encapsulation of catabolic cells, which consume lipids for heat production in intra-abdominal adipose tissue and increase energy dissipation in obese mice.