Xi'an Jiaotong University 10 articles published in JoVE Immunology and Infection A Catheter-Related Candida albicans Infection Model in Mouse Chen Yang1, Fei Mo2, Jiaxue Zhang1, Peipei Zhang1, Qingqing Li1, Jiye Zhang1 1 We establish a mouse model of C.albicans-associated catheter-related infection (CRI), in which biofilm forms on the catheter, and the interaction between C.albicans and host correlates well with the clinical CRI. This model helps screen therapies for C.albicans biofilm-associated CRI, laying a foundation for clinical transformation. Immunology and Infection A Periprosthetic Joint Candida albicans Infection Model in Mouse Chen Yang1, Jiaxue Zhang1, Fei Mo2, Peipei Zhang1, Qingqing Li1, Jiye Zhang1 1 Periprosthetic joint infection (PJI) caused by dangerous pathogens is common in clinical orthopedics. Existing animal models cannot accurately simulate the actual situation of PJI. Here, we established a Candida albicans biofilm-associated PJI mouse model to research and develop new therapeutics for PJI. Engineering Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics Wenwen Yuan1,2,3, Hang Yuan1, Sixuan Duan1,2, Ruiqi Yong1, Jia Zhu1,2,4, Eng Gee Lim1,2, Ivona Mitrovic2, Pengfei Song1,2 1School of Advanced Technology, Xi’an Jiaotong - Liverpool University, 2Department of Electrical Engineering and Electronics, University of Liverpool, 3 This protocol describes a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanofibrillated cellulose paper, achieving a minimum width of 200 µm. Medicine Orthopedic Robot-Assisted Femoral Neck System in the Treatment of Femoral Neck Fracture Yuxuan Cong*1, Pengfei Wen*2, Yongchao Duan3, Hai Huang1, Yan Zhuang1, Pengfei Wang1 1 This article introduces a method of robot-assisted orthopedic surgery for screw placement during the treatment of femoral neck fracture using the femoral neck system, which allows for more accurate screw placement, improved surgical efficiency, and fewer complications. Medicine Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease Shuchen Yu1, Yuchen Zhang1, Chunhui Zhu2, Huan Zhou2, Jin Liu2, Junyi Sun1, Ang Li1,2, Dandan Pei1,3 1Department of Periodontology, College of Stomatology, Xi’an Jiaotong University, 3 This article describes two protocols: 1) adjunctive diode laser therapy for treating periodontitis and 2) probiotic Lactobacillus therapy for treating peri-implant disease, with emphasis on the laser usage mode (inside or outside pocket), laser application regimen (single or multiple sessions), and a probiotic protocol of professional and home administration. Bioengineering Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC) Xingjin Jian*1,2, Xiaojie Guo*1,2,3, Jia Wang4, Zheng Lin Tan1,2,6, Xin-hui Xing1,2,5, Liyan Wang3, Chong Zhang1,2,5 1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4 This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption. Neuroscience Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice YangDong Wang1,2,3,4, DanYang Zhu5, BaoYue Liu1,2,3,6, Kiryl D. Piatkevich1,2,3 1School of Life Sciences, Westlake University, 2Westlake Laboratory of Life Sciences and Biomedicine, 3Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, 4 This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice. Neuroscience Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments Rui Yang1,2,3, Vann Bennett2,3 1 Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G. Medicine Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch Yaguang Li1, Junhong Liu2,3, Gaixia Liu1, Zhenhong Pan2,3, Mingxia Zhang1, Yao Ma4, Qingxia Wei1,5, Hongping Xia1,6, Rui Xue Zhang1,2, Junjun She1 1 The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease. Medicine A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction Lifei Yang*1,2, Jianwen Lu*1,2,3, Yue Wang1,2,3, Mei Zhang1,2,3, Yuan Shi4, Shasha Wei1,2, Peng Liu1,2,3, Zheng Wu3, Yi Lv1,2,3, Rongqian Wu1,2 1 The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.
