Xi'an Jiaotong University

6 articles published in JoVE

• Medicine

  
  Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease Shuchen Yu¹, Yuchen Zhang¹, Chunhui Zhu², Huan Zhou², Jin Liu², Junyi Sun¹, Ang Li^1,2, Dandan Pei^{1,3 1}Department of Periodontology, College of Stomatology, Xi’an Jiaotong University, ³ This article describes two protocols: 1) adjunctive diode laser therapy for treating periodontitis and 2) probiotic Lactobacillus therapy for treating peri-implant disease, with emphasis on the laser usage mode (inside or outside pocket), laser application regimen (single or multiple sessions), and a probiotic protocol of professional and home administration.

• Bioengineering

  
  Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC) Xingjin Jian*^1,2, Xiaojie Guo*^1,2,3, Jia Wang⁴, Zheng Lin Tan^1,2,6, Xin-hui Xing^1,2,5, Liyan Wang³, Chong Zhang^{1,2,5 1}Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, ²Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, ³Luoyang TMAXTREE Biotechnology Co., Ltd., ⁴ This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

• Neuroscience

  
  Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice YangDong Wang^1,2,3,4, DanYang Zhu⁵, BaoYue Liu^1,2,3,6, Kiryl D. Piatkevich^{1,2,3 1}School of Life Sciences, Westlake University, ²Westlake Laboratory of Life Sciences and Biomedicine, ³Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, ⁴ This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice.

• Neuroscience

  
  Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments Rui Yang^1,2,3, Vann Bennett^{2,3 1} Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

• Medicine

  
  Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch Yaguang Li¹, Junhong Liu^2,3, Gaixia Liu¹, Zhenhong Pan^2,3, Mingxia Zhang¹, Yao Ma⁴, Qingxia Wei^1,5, Hongping Xia^1,6, Rui Xue Zhang^1,2, Junjun She^{1 1} The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

• Medicine

  
  A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction Lifei Yang*^1,2, Jianwen Lu*^1,2,3, Yue Wang^1,2,3, Mei Zhang^1,2,3, Yuan Shi⁴, Shasha Wei^1,2, Peng Liu^1,2,3, Zheng Wu³, Yi Lv^1,2,3, Rongqian Wu^{1,2 1} The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.