Nantong University 4 articles published in JoVE Neuroscience In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos Jiaqi Wang*1,2, Rui Chai*3, Xiaoxia Fang2, Jiajia Gu4, Wanqing Xu2, Qi Chen1, Gang Chen2,3, Shunxing Zhu5, Yan Jin1 1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University This protocol describes how to obtain images by combining in situ hybridization and immunohistochemistry of zebrafish embryonic sections. In situ hybridization was performed prior to cryosectioning, followed by antibody staining. It is useful to detect the expression patterns of two genes in zebrafish if there is a paucity of antibodies. Medicine Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons Wei Feng Mao1, Ya Fang Wu2 1Department of Anatomy, Medical School, Nantong University, 2Department of Hand surgery, Affiliated Hospital of Nantong University Here, we present a “Q” suture technique that can be performed in tendon repair and its effects on the gap formation and tensile strength of the repaired tendons. Q suture is shown to be efficient in enhancing the tensile resistance and tendon repair strength. Neuroscience Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro Qianru He1, Fanhui Yu1, Yan Li1, Junjie Sun1, Fei Ding1 1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University Here, we present a method to purify fibroblasts and Schwann cells from sensory and motor nerves in vitro. Neuroscience An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation Zhe Zhao1, Haitao Wu1,2 1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University This protocol shows how to set up a reliable HFS method in mice. Neurons throughout the hippocampal dentate gyrus are electrically stimulated by HFS directly and indirectly in vivo. Neuronal activity and molecular signaling are examined by c-fos and Notch1 immunofluorescent staining, respectively; neurogenesis is quantified by bromodeoxyuridine labeling assay.
