The First Affiliated Hospital of Guangxi Medical University 1 article published in JoVE Biology Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse Zhen Wang1,4, Ramon Ocadiz-Ruiz1, Sinju Sundaresan1, Lin Ding1, Michael Hayes1, Nirakar Sahoo3, Haoxing Xu1,2, Juanita L. Merchant1,2,5 1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.
