First Affiliated Hospital of Jinan University

4 articles published in JoVE

• Medicine

  
  Establishment and Evaluation of a Porcine Vein Graft Disease Model Xiaohui Li*¹, Jinlin Hu*¹, Weijiang Tan*², Zhaoming Lin*¹, Caiyi Zhu², Chengfeng Huang¹, Jiawen Huang³, Yintian Liu¹, Qiuying Liao¹, Hua Lu¹, Xiaoshen Zhang^{1 1}Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University, ²Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, ³The First Affiliated Hospital of Jinan University In this protocol, novel pig vein bypass grafting was performed through a small incision in the left chest wall without cardiopulmonary bypass. A postoperative pathology study was done, which showed intimal thickening.

• Neuroscience

  
  Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation Qiwei Fan*¹, Xiaotan Zhang*², Yuan Wang³, Xiaoyu Wang^{1 1}Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, ²Department of Clinical Pathology, First Affiliated Hospital of Jinan University, ³Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly.

• Bioengineering

  
  Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System Yingwei Wang*^1,2, Cheng Lu*^1,2, Chengzhi He³, Baoxin Chen^1,2, Youling Zheng^1,2, Junming Zheng^1,2, Jianhua Zhang⁴, Zheng Wu^{1,2 1}Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, ²Department of Developmental and Regenerative Biology, Jinan University, ³Nansha College Preparatory Academy, ⁴Department of Cardiology, First Affiliated Hospital of Jinan University This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.

• Immunology and Infection

  
  A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice Ming Fu¹, Zefeng Lin², Huiting Lin², Yanlu Tong², Hezhen Wang², Hongjiao Chen², Yan Chen², Ruizhong Zhang^{2 1}First Affiliated Hospital of Jinan University, ²Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies.