2 articles published in JoVE
Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow Lining Wang1,2, Suyang Zheng1,2, Yang Guo1,2, Yalan Pan1,2, Jie Sun1,2, Weimin Xu1,2, Jinlan Lu3, Weidong Li3, Yong Ma1,2,4 1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.
Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography Jie Sun1, Guang Ming Yang2, Tao Tao1, Li Sha Wei1, Yang Pan2, Min Sheng Zhu1 1Model Animal Research Center and MOE Key Laboratory of Model Animal and Disease Study, Nanjing University, 2School of Pharmacy, Nanjing University of Chinese Medicine The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.