2 articles published in JoVE
Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis Ziming Chen*1,2, Zhantao Deng*1, Yuanchen Ma1, Junxing Liao1, Qingtian Li1, Mengyuan Li1, Hua Liu1, Ganghong Chen1, Chaoming Zeng1, Qiujian Zheng1 1Department of Orthopedics, Guangdong General Hospital (Guangdong Academy of Medical Sciences), 2Shantou University Medical College Knee osteoarthritis is frequently seen in the orthopedic department. We introduce in detail the entire knee osteoarthritis treatment process with platelet-rich plasma injection, including preparation, procedures, and evaluation.
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro Zhaofeng Jia1,2, Yujie Liang3, Bin Ma4,5, Xiao Xu2,6, Jianyi Xiong2, Li Duan2, Daping Wang1,2 1Guangzhou Medical University, 2 We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.