The First Affiliated Hospital of Nanchang University 2 articles published in JoVE Neuroscience Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons Mengye Zhu1, Daying Zhang1, Sicong Peng2, Nana Liu2, Jing Wu2, Haixia Kuang2, Tao Liu2,3 1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied. Medicine Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells Rui Liang*1, Wei Dong*1,3, Xiaopeng Shen1, Xiaoping Peng1,3, Angie G. Aceves1,2, Yu Liu1 1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.
