Yan Bian University Hospital 1 article published in JoVE Biology Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling Zai Hao Zhao1, Chun Li Jin1, Ji Hyun Jang1, Yu Na Wu1, Sung Joon Kim1, Hong Hua Jin2, Lan Cui2, Yin Hua Zhang1,2,3 1Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute, Seoul National University College of Medicine, 2Yan Bian University Hospital, 3Institute of Cardiovascular Sciences, University of Manchester This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.