3 articles published in JoVE
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane Moe Ishihara*1, Junhui Hu*1, Xiaoyu Zhang2, YongHyeon Choi3, Anthony Wong4, Celine Cano-Ruiz5, Rongwei Zhao6, Ping Tan7, Jonathan L. Tso1, Lily Wu1,8 1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Bioengineering, Hanyang University, 4Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 6School of Life Sciences, Beijing Normal University, 7Department of Urology, West China Hospital, Sichuan University, 8Department of Urology, David Geffen School of Medicine, University of California, Los Angeles Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. This protocol illustrates two novel animal models for the disease: the orthotopically implanted mouse model and the chicken chorioallantoic membrane model, both of which demonstrate lung metastasis resembling clinical cases.
Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation Wanting Chen1, Rui Chen1, Qinghua He1,2,3,4,5 1Faculty of Psychology, Southwest University, 2Key Laboratory of Cognition and Personality, Ministry of Education, Southwest University, 3Southwest University Branch, Collaborative Innovation Center of Assessment toward Basic Education Quality, Beijing Normal University, 4Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, 5Chongqing Collaborative Innovation Center for Brain Science Presented here is a protocol to achieve higher accuracy in determination of stimulation location combining a 3D digitizer with high-definition transcranial direct current stimulation.
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo Mikko Juusola1,2, An Dau2, Lei Zheng1, Diana Rien1,2 1National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, 2Department of Biomedical Science, The University of Sheffield Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.