Shanghai Institute Of Cardiovascular Diseases 2 articles published in JoVE Bioengineering Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution Nan Chen1,2, Kai Zhu1,2, Shiqiang Yan3,4, Junmin Li1,2, Tianyi Pan4, Mieradilijiang Abudupataer1,2, Fazle Alam4, Xiaoning Sun1,2, Li Wang3,4, Chunsheng Wang1,2 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College, Fudan University Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells. Medicine Three-Dimensional Printing of a Complex Aortic Anomaly Xiaoning Sun1,2, Kai Zhu1,2, Weijia Zhang1,2,3,4, Hongqiang Zhang1,2, Fazong Hu5, Chunsheng Wang1,2 1Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Disease, 3State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 4Institutes of Biomedical Sciences, Fudan University, 5Meditool Shanghai Enterprise Co., Ltd. Here, we present a protocol to use three dimensional printed models for pre-operative planning and intra-operative reorganization of complicated vascular locations when handling a congenital aortic anomaly.