Guangzhou Women and Children's Medical Center 4 articles published in JoVE Immunology and Infection A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia Xinrui Wang1,2, Ruizhong Zhang2, Zefeng Lin2, Ming Fu2, Yan Chen1,2 1School of Medicine, South China University of Technology, 2 We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments. Medicine International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure Jing Liu1,2, Dalibor Kurepa3, Francesco Feletti4,5, Almudena Alonso-Ojembarrena6, Jovan Lovrenski7, Roberto Copetti8, Erich Sorantin9, Javier Rodriguez-Fanjul10, Karishma Katti3, Andrea Aliverti4, Huayan Zhang11,12, Misun Hwang13, Tsu F. Yeh14, Cai-Bao Hu15, Xing Feng16, Ru-Xin Qiu1,2, Jing-Han Chi17, Li-Li Shang18, Guo-Rong Lyu19, Shao-Zheng He20, Yan-Fen Chai21, Zhan-Jun Qiu22, Hai-Ying Cao2,23, Yue-Qiao Gao1,2, Xiao-Ling Ren1,2, Guo Guo1,24, Li Zhang1,2, Ying Liu1,2, Wei Fu1,2, Zu-Lin Lu1,2, Hong-Lei Li1,2 1Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, 2The National Neonatal Lung Ultrasound Training Base, 3 Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of pneumothorax. Immunology and Infection Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster Shuo Yang*1,2,3, Yaya Zhao*1,2, Junjing Yu4, Zhiqin Fan2, Si-tang Gong1, Hong Tang1,2, Lei Pan1,2,3 1 This protocol describes how to establish viral infection in vivo in Drosophila melanogaster using the nano-injection method and basic techniques to analyze virus-host interaction. Medicine Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus Jing Liu1,2, Roberto Copetti3, Erich Sorantin4, Jovan Lovrenski5, Javier Rodriguez-Fanjul6, Dalibor Kurepa7, Xing Feng8, Luigi Cattaross9, Huayan Zhang10,11, Misun Hwang12, Tsu F. Yeh13,14, Yisrael Lipener7, Abhay Lodha15, Jia-Qin Wang16, Hai-Ying Cao2,17, Cai-Bao Hu2,18, Guo-Rong Lyu19, Xin-Ru Qiu1,2, Li-Qun Jia20, Xiao-Man Wang20, Xiao-Ling Ren1,2, Jiu-Ye Guo1,2, Yue-Qiao Gao1,2, Jian-Jun Li1,2, Ying Liu1,2, Wei Fu1,2, Yan Wang21, Zu-Lin Lu1,2, Hua-Wei Wang8, Li-Li Shang22 1Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, 2The Neonatal Lung Ultrasound Training Base, Chinese College of Critical Ultrasound, 3Emergency Department, Cattinara University Hospital, 4Division of Pediatric Radiology, Department of Radiology, Medical University Graz, 5Faculty of Medicine, University of Novi Sad, Radiology Department, Institute for Children and Adolescents Health Care of Vojvodina, 6Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, 7Department of Neonatology, Children's Hospital of Soochow University, 9Department of Neonatology, Udine University Hospital, 10Division of Neonatology, Children's Hospital of Philadelphia, 12Intensive Care Unit, Zhejiang Hospital, 19Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, 20Department of Neonatology and NICU, Tai'an City Central Hospital of Shandong Province, 22Department of Intensive Care Unit, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.
