Peking University Third Hospital 7 articles published in JoVE Medicine Rotating the Intraocular Lens to Prevent Posterior Capsular Opacification in Cataract Surgeries Di Zhang*1,2, Ziyuan Liu*1,2, Hongyuan Cai1,2, Haikun Wang1,2, Xiaoyong Chen1,2, Chun Zhang1,2 1Department of Ophthalmology, Peking University Third Hospital, 2Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital The present protocol describes removing residual epithelial cells by rotating the intraocular lens in extracapsular cataract surgery without extra tools for preventing posterior capsular opacification. Medicine Iris Fixation via External Pentagram Suturing Xiang Fan1,2, Yansheng Hao1,3, Ying Chu1,2, Jing Hong1,2 1Department of Ophthalmology, Peking University Third Hospital, 2Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, 3Xian Aier Eye Hospital The present protocol describes how a 10-0 polypropylene suture crosses the anterior surface of the iris, forming a pentagram to prevent both iris and pupil from moving toward the cornea. This can be combined with subsequent keratoplasty to cure bullous keratopathy with extensive anterior synechia of the iris. Medicine Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients Yuexin Wang*1, Yining Guo*1, Shanshan Wei2, Yifei Yuan1, Tingyi Wu1, Yu Zhang1, Yueguo Chen1, Xuemin Li1 1Department of Ophthalmology, Beijing Key Laboratory of Restoration of Damaged Ocular Nerves, Peking University Third Hospital, 2Beijing Tongren Eye Center, Beijing Tongren Hospital The present research demonstrates a method to accurately examine dynamic visual acuity (DVA) in myopic subjects with eyeglass correction. Further analysis indicated that the closer the refraction state to emmetropia, the better the eyeglass-corrected binocular DVA is at both 40 and 80 degrees per second. Genetics Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis Jin Huang*1,2,3,4, Yaxin Yao*5, Jialin Jia1,2,3,4, Xiaohui Zhu1,2,3,4, Jieliang Ma5, Jing Wang5, Ping Liu1,2,3,4, Sijia Lu5 1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd. The present study reports a protocol for chromosome screening of human embryos that uses spent culture medium, which avoids embryo biopsy and enables chromosome ploidy identification using NGS. The present article presents the detailed procedure, including the preparation of culture medium, whole genome amplification (WGA), next-generation sequencing (NGS) library preparation, and data analysis. Medicine OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery Tao Liu*1,2,3,4,5, Xueling Song*1,2,3,4,5, Xiaoying Zheng*1,2,3,4,5, Liying Yan1,2,3,4,5, Caihong Ma1,2,3,5, Rong Li1,2,3,4,5, Jie Yan1,2,3,4,5, Jie Qiao1,2,3,4,5 1Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology, 3Key Laboratory of Assisted Reproduction, Ministry of Education, 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, 5Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation. Neuroscience Assessing Early Stage Open-Angle Glaucoma in Patients by Isolated-Check Visual Evoked Potential Xiang Fan1,2, Lingling Wu1,2, Aihua Ding1,2 1Department of Ophthalmology, Peking University Third Hospital, 2Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital The isolated-check visual evoked potential (icVEP) method is implemented here to assess the magnocellular ON pathway that is initially damaged in glaucoma. The study shows standard operative procedures using icVEP to obtain reliable results. It is proved to serve as a useful objective diagnosing technology for the early detection of glaucoma. Medicine Targeting Gray Rami Communicantes in Selective Chemical Lumbar Sympathectomy Wen-Hui Wang1, Long Zhang2,3, Guo-Xiang Dong2, Jun Zhao2, Xuan Li2,3 1Department of Dermatology, Peking University Third Hospital, 2Department of Interventional Radiology and Vascular Surgery, Peking University Third Hospital, 3Wound Healing Center, Peking University Third Hospital We present a protocol for selective chemical lumbar sympathectomy (CLS), which inactivates only gray rami communicantes and not the sympathetic trunk. Selective CLS can help achieve therapeutic efficacy in vasodilation, sweat reduction, and pain relief that are comparable to conventional CLS, and serious complications, particularly ureteropelvic damages, can be reduced.
