The Second Hospital of Dalian Medical University 1 article published in JoVE Immunology and Infection Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis Yu Zhang *1, Guan Wang*1, Jia Lu1, Li-ming Xu2, Jun-yu Xiong1 1Anesthesiology Department, The Second Hospital of Dalian Medical University, 2Scientific Research Center, The Second Hospital of Dalian Medical University Here, we present a protocol to assess mouse peritoneal macrophage phagocytosis using enhanced green fluorescence protein-expressing Escherichia coli.