UCLA Institute of Advancement (Suzhou) 1 article published in JoVE Biochemistry Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers Yi-Xin Huo1,2, Bo Zheng1, Ning Wang1, Yunpeng Yang1, Xinxin Liang1, Xiaoyan Ma1 1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, 2UCLA Institute for Technology Advancement (Suzhou) This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.