Yangzhou University 9 articles published in JoVE Immunology and Infection A Mouse Ear Model for Allergic Contact Dermatitis Evaluation Haifeng Shao1,2,3,4, Jialin Deng2,3,4, Yanqing Liu1,2,3,4, Niuniu Yang1,2,3,4 1Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, 2Department of Traditional Chinese and Western Medicine, Medical College, Yangzhou University, 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, 4Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University Here, we describe the methods for inducing allergic contact dermatitis in mouse ears by 1-fluoro-2,4-dinitrobenzene (DNFB) and how to evaluate the severity of allergic contact dermatitis. Biology AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants Ahmad Zada*1, Imran Khan*1, Min Zhang1, Yuting Cheng1, Xueyun Hu1 1College of Bioscience and Biotechnology, Yangzhou University Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique. Developmental Biology In Ovo Intravascular Injection in Chicken Embryos Kai Jin1,2, Jing Zhou1,2, Gaoyuan Wu1,2, Ziyi Lian1,2, Zongyi Zhao1,2, Shujian Zhou1,2, Chen Chen1,2, Hongyan Sun1,2, Yingjie Niu1,2, Qishenng Zuo1,2, Yani Zhang1,2, Jiuzhou Song3, Guohong Chen1,2, Bichun Li1,2,4 1Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, 2Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, 3Animal & Avian Sciences, University of Maryland, College Park, 4College of Biotechnology, Jiangsu University of Science and Technology The overall goal of this paper is to describe how to perform in ovo intracellular injection of exogenous materials into chicken embryos. This approach is very useful to study the developmental biology of chicken embryos. Biochemistry The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination Ziyi Wang1,2, Qinghua Liu3,4, Liang Wang3,5, Robert G. Gilbert1,2,6, Mitchell A. Sullivan7 1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial. Immunology and Infection Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium Pengpeng Xia1,2,3, Siqi Lian1,2,3, Yunping Wu1,2,3, Li Yan1,2,3 1College of Veterinary Medicine (Institute of comparative medicine), Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University The recombinant antibody protein expressed in pIRES2-ZSGreen1-rAbs-APN-CHO cells and monoclonal antibodies produced using traditional hybridoma technology can recognize and bind to the porcine aminopeptidase N (APN) protein. Immunology and Infection A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis Xia Meng*1,2, Weiwei Cui*1, Xianchen Meng1, Jianye Wang1,2, Jinqiu Wang3, Guoqiang Zhu1,2 1College of Veterinary Medicine, Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Department of Animal Husbandry and Veterinary Medicine, Beijing Agricultural Vocational College An λ-Red-mediated recombination system was used to create a deletion mutant of a small non-coding RNA micC. Biology A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications Jiajing Qiu*1,2, Yinhui Ren*1,2, Lingxiao Zhao1,2, Biao Zhang1,2, Cunxu Wei1,2 1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, 2Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University This technique allows for the fast and simple preparation of whole-seed-sized resin section for the observation and analysis of cells, starch granules, and protein bodies in different regions of the seed. Biology Measuring Crop Motility and Food Passaging in Drosophila Jiadong Cai*1,2, Junmeng Xi*1,2, Youheng Wei1,2 1Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, 2College of Bioscience and Biotechnology, Yangzhou University The goal of this protocol is to measure crop contraction and quantify food distribution in the Drosophila gut. Biochemistry Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade Zhiping Zhang1, Shuhang Fan1, Zhiqiang Chen1, Yanzhi He1, Mengfei Huang1, Li Ding1, Yanyan Zhang5, Lin Chen1, Xinyue Zhang1,2,3,4 1College of Bioscience and Biotechnology, Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center, Yangzhou University The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.