Guangzhou Institute of Respiratory Health 1 article published in JoVE Medicine Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE) Zhifeng Huang*1, Wenting Luo*1, Xiangwei Zou1, Xiaoqing Liu1, Chuanxu Cai1, Zehong Wu1, Haisheng Hu1, Baoqing Sun1 1Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University This paper presents a protocol for detecting serum-specific immunoglobulin E with a microfluidic cartridge-based chemiluminescence system and the evaluation of its usefulness in allergy diagnoses.