Jiaxing Academy of Agricultural Sciences 1 article published in JoVE Genetics Identifying Mutations by High Resolution Melting in a TILLING Population of Rice Shan Li1, Ying-Ping Yu1, Song-Mei Liu1, Hao-Wei Fu2, Jian-Zhong Huang3, Qing-Yao Shu1, Yuan-Yuan Tan4 1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).
