Shanghai Jiao Tong University

3 articles published in JoVE

• Bioengineering

  
  Viscoelastic Characterization of Soft Tissue-Mimicking Gelatin Phantoms using Indentation and Magnetic Resonance Elastography Yuan Feng¹, Suhao Qiu¹, Yu Chen¹, Runke Wang¹, Zhao He¹, Linghan Kong¹, Yi Chen¹, Shengyuan Ma^{1 1}School of Biomedical Engineering, Shanghai Jiao Tong University This article presents a demonstration and summary of protocols of making gelatin phantoms that mimic soft tissues, and the corresponding viscoelastic characterization using indentation and magnetic resonance elastography.

• Biology

  
  Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion Chao E*¹, Liqiang Dai*^1,2, Jiaqi Tian^3,4, Lin-Tai Da⁴, Jin Yu^{5,6,7 1}Beijing Computational Science Research Center, ²Shenzhen JL Computational Science and Applied Research Institute, ³School of Medical Informatics and Engineering, Xuzhou Medical University, ⁴Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, ⁵Department of Physics and Astronomy, University of California, Irvine, ⁶Department of Chemistry, University of California, Irvine, ⁷NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine The goal of this protocol is to reveal structural dynamics of one-dimensional diffusion of protein along DNA, using a plant transcription factor WRKY domain protein as an exemplary system. To do this, both atomistic and coarse-grained molecular dynamics simulations along with extensive computational samplings have been implemented.

• Developmental Biology

  
  Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue Yiding Qi*¹, Xiang Miao*², Lian Xu³, Mengxia Fu³, Shi Peng⁴, Kailei Shi⁵, Jun Li⁴, Maoqing Ye⁵, Ruogu Li^{1 1}Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, ²Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, ³Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ⁴Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ⁵Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre^+/-;Rosa26^RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.