Guizhou Normal University 2 articles published in JoVE Biology Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks Jing Tang*1,2, Xinzhu He*1, Ming Tang1, Xiaorong Xu2, Ximin Zhang1 1Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, 2State Key Laboratory of Plant Physiology and Development in Guizhou Province, Guizhou Normal University Network analysis was applied to evaluate the association of various ecological microbial communities, such as soil, water and rhizosphere. Presented here is a protocol on how to use the WGCNA algorithm to analyze different co-occurrence networks that may occur in the microbial communities due to different ecological environments. Genetics Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum) Yaolei Mi1,2, Zhihui Zhu3, Guangtao Qian3, Yu Li4, Xiangxiao Meng1, Jianping Xue3, Qingfu Chen5, Wei Sun1, Yuhua Shi1,2 1Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 2Artemisinin Research Center, China Academy of Chinese Medical Sciences, 3College of Life Science, Huaibei Normal University, 4Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, 5Research Center of Buckwheat Industry Technology, Guizhou Normal University We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.
