The General Hospital of Western Theater Command 1 article published in JoVE Cancer Research Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice Lisha Wang1, Zhiming Wang1, Junyi Guo2, Huayu Lin1, Shuqiong Wen2, Qiao Liu1, Yiding Li3, Qing Wu1, Leiqiong Gao1, Xiangyu Chen1, Luoyingzi Xie1, Qin Tian1, Jianfang Tang1, Zhirong Li1, Li Hu1, Juan Wang4, Lifan Xu1, Qizhao Huang5, Lilin Ye1 1Institute of Immunology, Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital, Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, 5Cancer Center, The General Hospital of Western Theater Command Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.