Guangzhou Darui Reproduction Technology Co., Ltd.
1 article published in JoVE
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform Chengming Xu*1, Riqing Wei*2, Hui Lin1, Leiyu Deng1, Li Wang3, Deyang Li4, Honghui Den5, Wensong Qin1, Ping Wen1, Ying Liu1, Yingsong Wu2, Qiang Ma2, Jinliang Duan1 1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd. The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results.