Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes 1 article published in JoVE Bioengineering Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination Bangzhu Chen1,2, Qinrong Lin1,2, Nengqing Liu1,2, Diyu Chen1,2, Yinghui Zhang3, Xiaofang Sun1,2 1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences, Wuyi University Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.
