2 articles published in JoVE
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus Yuanchao Zhan1, Yaxin Liu1, Qinglu Zeng1,2,3 1Department of Ocean Science, Hong Kong University of Science and Technology, 2Division of Life Science, Hong Kong University of Science and Technology, 3HKUST Shenzhen Research Institute Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.
Microfluidic Mixers for Studying Protein Folding Steven A. Waldauer1, Ling Wu1, Shuhuai Yao2, Olgica Bakajin3, Lisa J. Lapidus1 1Department of Physics and Astronomy, Michigan State University, 2Department of Mechanical Engineering, Hong Kong University of Science and Technology, 3Center for Biophotonics, University of California, Davis In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).