Hong Kong Baptist University

3 articles published in JoVE

• Neuroscience

  
  Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor Jason Cho Wai Lo¹, Wee Lin Wong¹, Catherine Hong Huan Hor^{1 1}Department of Chemistry, Faculty of Science, Hong Kong Baptist University Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.

• Behavior

  
  Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running Wenjing Quan¹, Meizi Wang², Gongju Liu², Gusztáv Fekete³, Julien S. Baker⁴, Feng Ren¹, Yaodong Gu^{1 1}Faculty of Sports Science, Ningbo University, ²Faculty of Engineering, University of Pannonia Veszeprem, ³Savaria Institute of Technology, Eötvös Loránd University, ⁴Department of Sport, and Physical Education, Hong Kong Baptist University This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively.

• Developmental Biology

  
  A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate Po Ying Poon¹, Patrick Ying Kit Yue¹, Ricky Ngok Shun Wong^{1 1}Department of Biology, Hong Kong Baptist University Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.