Hong Kong Baptist University
3 articles published in JoVE
Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor Jason Cho Wai Lo1, Wee Lin Wong1, Catherine Hong Huan Hor1 1Department of Chemistry, Faculty of Science, Hong Kong Baptist University Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.
Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running Wenjing Quan1, Meizi Wang2, Gongju Liu2, Gusztáv Fekete3, Julien S. Baker4, Feng Ren1, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2Faculty of Engineering, University of Pannonia Veszeprem, 3Savaria Institute of Technology, Eötvös Loránd University, 4Department of Sport, and Physical Education, Hong Kong Baptist University This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively.
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate Po Ying Poon1, Patrick Ying Kit Yue1, Ricky Ngok Shun Wong1 1Department of Biology, Hong Kong Baptist University Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.