3 articles published in JoVE
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells Shino Mitsunaga1, Keiko Shioda1, Kurt J. Isselbacher1, Jacob H. Hanna2, Toshi Shioda1 1Center for Cancer Research, Massachusetts General Hospital, 2Department of Molecular Genetics, Weizmann Institute of Science Primordial germ cells (PGCs) are common precursors of both sperm and eggs. Human embryonic PGCs are specified from pluripotent epiblast cells through interactions of cytokines. Here, we describe a 13-day protocol of inducing human cells transcriptomally resembling PGCs at the surface of embryoid bodies from primed-pluripotency induced pluripotent stem cells.
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments Rinat Arbel-Goren1, Yonatan Shapira1, Joel Stavans1 1Department of Physics of Complex Systems, Weizmann Institute of Science This manuscript describes a method for labeling individual messenger RNA (mRNA) transcripts with fluorescently-labeled DNA probes, for use in single-molecule fluorescence in situ hybridization (smFISH) experiments in E. coli. smFISH is a visualization method that allows the simultaneous detection, localization, and quantification of single mRNA molecules in fixed individual cells.
External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures Shani Stern1, Assaf Rotem2, Yuri Burnishev3, Eyal Weinreb3, Elisha Moses3 1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Department of Physics and SEAS, Harvard University, 3Department of Physics of Complex Systems, Weizmann Institute of Science Neuronal cultures are a good model for studying emerging brain stimulation techniques via their effect on single neurons or a population of neurons. Presented here are different methods for stimulation of patterned neuronal cultures by an electric field produced directly by bath electrodes or induced by a time-varying magnetic field.