National Institute of Immunology View Institution's Website 4 articles published in JoVE Medicine Microdissection of the Rodent Eye K. Varsha Mohan1, Prakriti Sinha1, Bharati Swami1, Abaranjitha Muniyasamy1, Perumal Nagarajan1, Pramod Upadhyay1 1National Institute of Immunology This paper presents a protocol for ocular micro-dissection in rodents. The process involves the enucleation of the eyeball along with the nictitating membrane (i.e., the third eyelid). This is then followed by the separation of the posterior and anterior eye cups. Medicine Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen Preeti Sahay1, Kshama Jain1, Prakriti Sinha1, Barun Das1, Alaknanda Mishra1, Ashwani Kesarwani1, Parul Sahu1, K. Varsha Mohan1, M.J. Mahesh Kumar2, Perumal Nagarajan1, Pramod Upadhyay1 1National Institute of Immunology, 2Center for Cellular and Molecular Biology The acute liver failure animal model developed in the current study presents a feasible alternative for the study of potential therapies. The current model employs the combined effect of physical and drug-induced hepatic injury and provides a suitable time window to study the potential of novel therapies. Medicine Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice Barun Das1, Jashdeep Bhattacharjee1, Preeti1, Alaknanda Mishra1, Kshama Jain1, Srikanth Iyer1, Ashwani Kesarwani1, Parul Sahu1, Prakriti Sinha1, Perumal Nagarajan1, Pramod Upadhyay1 1National Institute of Immunology We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells. Medicine Protocol for Long Duration Whole Body Hyperthermia in Mice Vikas Duhan*1, Neha Joshi*1, P. Nagarajan2, Pramod Upadhyay1 1Product Development Cell, National Institute of Immunology, 2Small Animal Facility, National Institute of Immunology This paper describes a protocol for whole body hyperthermia in mice that can stimulate fever like conditions up to 12-24 hr.