3 articles published in JoVE
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs Varsha Tirumalai1,2, Melvin Prasad1, P. V. Shivaprasad1 1National Centre for Biological Sciences, Tata Institute of Fundamental Research, 2SASTRA University This method demonstrates use of the northern hybridization technique to detect miRNAs from total RNA extract.
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish Elaine L. Welch*1, Celeste C. Eno*1, Sreelaja Nair2, Robin E. Lindeman3, Francisco Pelegri1 1Laboratory of Genetics, University of Wisconsin-Madison, 2Department of Biological Sciences, Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development, University of Minnesota An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.
Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish Sudip Mondal1, Shikha Ahlawat1, Sandhya P. Koushika1,2 1Neurobiology, NCBS-TIFR, 2Department of Biological Sciences, TIFR A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.