CSIR-National Chemical Laboratory 2 articles published in JoVE Biology Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera Sharada D. Mohite1,2, Yogita P. Patil1,2, Vikram J. Nichit1,2, Rakesh S. Joshi1,2 1Biochemical Sciences Division, CSIR-National Chemical Laboratory, 2Academy of Scientific and Innovative Research (AcSIR) This protocol describes the obligate feeding assay to evaluate the potentially toxic effect of a phytochemical on the lepidopteran insect larvae. This is a highly scalable insect bioassay, easy to optimize the sublethal and lethal dose, deterrent activity, and physiological effect. This could be used for screening eco-friendly insecticides. Environment Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance Santosh G. Tupe1, Ejaj K. Pathan1, Mukund V. Deshpande1 1Biochemical Sciences Division, CSIR-National Chemical Laboratory Here, we report the different stages involved in the knowledge-based development of an effective mycoinsecticide, including the isolation, identification, screening, and selection of the "best-fit" entomopathogenic fungus, Metarhizium anisopliae, for the control of insect pests in agriculture.
