Indian Institute of Science 5 articles published in JoVE Immunology and Infection Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice Avik Chattopadhyay1, Joel P. Joseph2, Sai Shyam3, Dipankar Nandi1 1Department of Biochemistry, Indian Institute of Science, 2Center for BioSystems Science and Engineering, Indian Institute of Science, 3Undergraduate Program, Indian Institute of Science This protocol describes the induction of Gram-negative monobacterial sepsis in a mouse model system. The model is useful in investigating the inflammatory and lethal host responses during sepsis. Biochemistry Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes Satyaghosh Maurya1, Vishwesh Haricharan Rai1, Aditya Upasani1, Saurabh Umrao1,2, Diksha Parwana1, Rahul Roy1,3 1Department of Chemical Engineering, Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab, University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering, Indian Institute of Science Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented. Biology The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking Anshul Milap Bhatt1, Subba Rao Gangi Setty1 1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore KA 560012, India Recycling endosomes are part of the endosomal tubular network. Here we present a method to quantify the dynamics of recycling endosomes using GFP-STX13 as an organelle marker. Immunology and Infection User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy Anil Kumar1, Surekha P.1, Sahil Gulati2, Somnath Dutta1 1Molecular Biophysics Unit, Indian Institute of Science, 2Gatan Inc. Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions. Engineering A Fabrication and Measurement Method for a Flexible Ferroelectric Element Based on Van Der Waals Heteroepitaxy Jie Jiang*1, Yugandhar Bitla*2, Qiang-xiang Peng1, Yi-Chun Zhou1, Ying-Hao Chu3 1Key Laboratory of Low Dimensional Materials and Application Technology of Ministry of Education, Xiangtan University, 2Department of Physics, Indian Institute of Science, 3Department of Materials Science and Engineering, National Chiao Tung University In this paper, we present a protocol to directly grow an epitaxial yet flexible lead zirconium titanate memory element on muscovite mica.
