Rajiv Gandhi Centre for Biotechnology 1 article published in JoVE Medicine Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model Ajith Kumar GS*1, Binil Raj*1, Santhosh Kumar S2, Sanjay G3, Chandrasekharan Cheranellore Kartha1 1Cardio Vascular Disease Biology, Rajiv Gandhi Centre for Biotechnology, 2Animal Research Facility, Rajiv Gandhi Centre for Biotechnology, 3Cardiology Department, Sree Chitra Tirunal Institute for Medical Sciences & Technology We describe a stepwise procedure for creating pressure overload and left ventricular hypertrophy in Wistar rats by constriction of the ascending aorta using a small metallic clip. This model is extensively used for studying remodeling changes during cardiac hypertrophy and for identifying and evaluating strategies for regression of such changes.
