The University of Trans-Disciplinary Health Sciences and Technology 1 article published in JoVE Developmental Biology In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development Nishant Singh*1,2, Anubhav Prakash*1, Suraj Ranganath Chakravarthy*1, Raman Kaushik*1, Raj K. Ladher1 1National Centre for Biological Sciences, Tata Institute of Fundamental Research, 2The University of Trans-Disciplinary Health Sciences and Technology The chick is a cost-effective, accessible, and widely available model organism for a variety of studies. Here, a series of protocols is detailed to understand the molecular mechanisms underlying avian inner ear development and regeneration.