Institute for Advanced Studies in Basic Sciences (IASBS) 1 article published in JoVE Biochemistry Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils Ramin Zadali1, Ebrahim Rostampour Ghareghozloo*1, Mohammad Ramezani*1, Vahid Hassani*1, Yasin Rafiei*1, Shiva Mohammadi Chiyaneh1, Ali Akbar Meratan1 1Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran Provided here is a protocol for investigating the interactions between native form, prefibrillar, and mature amyloid fibrils of different peptides and proteins with mitochondria isolated from different tissues and various areas of the brain.