National Institute of Agrobiological Sciences (Japan)View Institution's Website
1 article published in JoVE
A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells Hiroshi Kitani1, Takato Takenouchi1, Mitsuru Sato1, Miyako Yoshioka2, Noriko Yamanaka2 1Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan, 2Safety Research Team, National Institute of Animal Health, Tsukuba, Japan A novel method to obtain macrophages from primary culture of rat liver cells is described. This method utilizes the proliferation of macrophages in the culture, followed by shaking of culture flasks and purification by selective attachment to plastic dishes. This technique efficiently provides liver macrophages without complex equipment and skills.