Kyoto Prefectural University of Medicine View Institution's Website 4 articles published in JoVE Developmental Biology In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells Chuang-Yu Lin1, Michiko Yoshida1,2, Li-Tzu Li3, Megumu K. Saito1 1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy. Bioengineering A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance Ryohei Hirose1,2, Tomo Daidoji2, Yuji Naito1, Osamu Dohi1, Naohisa Yoshida1, Hiroaki Yasuda1, Hideyuki Konishi1, Takaaki Nakaya2, Yoshito Itoh1 1Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 2Department of Infectious Diseases, Graduate School of Medical Science, Kyoto Prefectural University of Medicine We developed a new ex vivo model that applies constant tension to the porcine gastric specimen. This development made it possible to evaluate the performance (the height and duration of the submucosal elevation) of various SIMs accurately. The detailed setup methodology of this new model is explained. Medicine Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach Toshiaki Numajiri1, Daiki Morita2, Hiroko Nakamura3, Ryo Yamochi1, Shoko Tsujiko4, Yoshihiro Sowa1 1Department of Plastic and Reconstructive Surgery, Kyoto Prefectural University of Medicine, 2Department of Plastic and Reconstructive Surgery, Showa University Fujigaoka Hospital, 3Department of Plastic and Reconstructive Surgery, Fukuchiyama City Hospital, 4Department of Plastic and Reconstructive Surgery, Saiseikai Shiga Hospital Methods for designing a computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guide are shown. Cutting planes are separated, united, and thickened to easily visualize the necessary bone transfer. These designs can be three-dimensional printed and checked for accuracy. Behavior Clinical-oriented Three-dimensional Gait Analysis Method for Evaluating Gait Disorder Masahiko Mukaino1, Kei Ohtsuka2, Hiroki Tanikawa2, Fumihiro Matsuda2, Junya Yamada3, Norihide Itoh4, Eiichi Saitoh1 1Department of Rehabilitation Medicine, School of Medicine, Fujita Health University, 2Faculty of Rehabilitation, School of Health Science, Fujita Health University, 3Department of Rehabilitation, Fujita Health University Hospital, 4Department of Advanced Rehabilitation Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine In this study, a clinician-friendly three-dimensional gait analysis method, which was designed to be performed in the rehabilitation clinic, is presented. The method consists of a simplified measurement method and intuitive figures to facilitate clinicians' understanding of the results.
