Okinawa Institute of Science and Technology (OIST) View Institution's Website 4 articles published in JoVE Neuroscience In vivo Calcium Imaging in Mouse Inferior Olive Da Guo1, Ayşen Gürkan Özer1, Marylka Yoe Uusisaari1 1Neuronal Rhythms in Movement unit, OIST We present a protocol to expose the brainstem of adult mouse from the ventral side. By using a gradient-refractive index lens with a miniature microscope, calcium imaging can be used to examine the activity of inferior olive neural somata in vivo. Environment Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica Aki Masunaga1, Andrew W. Liu1, Yongkai Tan1, Andrew Scott1, Nicholas M. Luscombe1,2,3 1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment, University College London Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions. Behavior New Variations for Strategy Set-shifting in the Rat Sho Aoki1, Andrew W. Liu1, Aya Zucca1, Stefano Zucca1, Jeffery R. Wickens1 1Neurobiology Research Unit, Okinawa Institute of Science and Technology Set-shifting, a form of behavioral flexibility, requires an attentional shift from one stimulus dimension to another. We extended an established rodent set-shifting task1 by requiring attention to different stimuli according to context. The task was combined with specific lesions to identify neuron subtypes underlying a successful shift. Neuroscience C. elegans Tracking and Behavioral Measurement Jirapat Likitlersuang1, Greg Stephens2,3, Konstantine Palanski1, William S. Ryu1,4 1Donnelly Centre, University of Toronto, 2Department of Physics and Astronomy, Vrije Universiteit, 3Okinawa Institute of Science and Technology, 4Department of Physics, University of Toronto We have developed a video-rate tracking microscope system that can record and quantify C. elegans behavior at high resolution and high speeds. We have also developed computational methods to reduce the dimensionality of the worm images to a fundamental set of measurements that completely describe the shape of the worm.
