2 articles published in JoVE
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon Atsushi Matsuda1,2, Takako Koujin1, Lothar Schermelleh3, Tokuko Haraguchi1,2, Yasushi Hiraoka1,2 1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes Naoki Ihara1, Yuji Ikegaya1,2, Haruki Takeuchi1,3 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST) Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.