3 articles published in JoVE
Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib Toshimitsu Yamaoka1, Motoi Ohba1, Yuki Matsunaga2, Junji Tsurutani1, Tohru Ohmori3 1Advanced Cancer Translational Research Institute, Showa University, 2Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, 3Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine A method for establishing afatinib-resistance cell lines from lung adenocarcinoma PC-9 cells was developed, and resistant cells were characterized. The resistant cells can be used to investigate epidermal growth factor receptor tyrosine kinase inhibitor-resistance mechanisms, applicable for patients with non-small cell lung cancer.
Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure Toshimitsu Yamaoka1, Motoi Ohba1, Satoru Arata1,2, Tohru Ohmori1 1Institute of Molecular Oncology, Showa University, 2Center for Biotechnology, Showa University An in vitro method for establishing dual resistance to an EGFR-TKI and a MET-TKI in cancer cells is described. This method is useful for developing treatments for patients with EGFR-mutations, who exhibit disease progression despite EGFR-TKI treatment with MET-amplification. It can also be modified for inhibitors targeting other molecules.
2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition Kenji Ikeya1, Miya Fukunishi1, Fuminori Iwasa1, Yuuki Inoue2, Kazuhiko Ishihara2, Kazuyoshi Baba1 1Department of Prosthodontics, School of Dentistry, Showa University, 2Department of Materials Engineering, School of Engineering, The University of Tokyo Removable poly(methyl methacrylate) (PMMA) dentures are prone to bacterial adherence and plaque formation. Denture plaque-associated infection is a source of serious dental and medical complications in the elderly. This paper introduces a novel protocol to treat PMMA dentures with 2-methacryloyloxyethyl phosphorylcholine polymer, poly(MPC-co-BMA-co-MPAz), to suppress plaque deposition on PMMA dentures.