Gunma University 5 articles published in JoVE Neuroscience Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons Noriko Koganezawa1, Reiko T. Roppongi2, Yuko Sekino3,4, Izuo Tsutsui3, Ayaka Higa5, Tomoaki Shirao1,5 1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc. A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate. Behavior Measurement of Spatial Stability in Precision Grip Ryoto Teshima1, Naoto Noguchi2, Ryota Fujii3, Ken Kondo4, Koji Tanaka4, Bumsuk Lee4 1Division of Rehabilitation Service, Geriatrics Research Institute and Hospital, 2Faculty of Rehabilitation, Gunma University of Health and Welfare, 3Department of Rehabilitation, Gunma Chuo Hospital, 4Graduate School of Health Sciences, Gunma University The goal of this protocol is to measure the center of pressure (COP) replacement using a high spatial resolution sensor sheet to reflect the spatial stability in a precision grip. The use of this protocol could contribute to greater understanding of the physiology and pathophysiology of grasping. Engineering A Polymer-based Piezoelectric Vibration Energy Harvester with a 3D Meshed-Core Structure Takuya Tsukamoto1, Yohei Umino1, Kotaro Hashikura1, Sachie Shiomi1, Kou Yamada1, Takaaki Suzuki1,2 1Gunma University, 2JST PRESTO In this study, we fabricated a flexible 3D mesh structure and applied it to the elastic layer of a bimorph cantilever-type vibration energy harvester for the purpose of lowering resonance frequency and increasing output power. Engineering Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions Kentaro Doi1, Fumika Nito1, Ayako Yano2, Ryo Nagura1, Satoyuki Kawano1 1Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, 2Division of Mechanical Science and Technology, Faculty of Science and Technology, GUNMA University The rectification of ion transport pathways is an effective method to generate one-directional ion-dragged electrohydrodynamic flows. By setting an ion-exchange membrane in a flow channel, an electrically polarized condition is generated and causes a liquid flow to be driven when an electric field is externally applied. Cancer Research One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy Daijiro Kobayashi1, Atsushi Shibata2, Takahiro Oike1, Takashi Nakano1 1Department of Radiation Oncology, Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.
