2 articles published in JoVE
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model Yuka Kato1, Takanori Iwata2, Kaoru Washio2, Toshiyuki Yoshida2, Hozue Kuroda2, Shunichi Morikawa3, Mariko Hamada1, Kazuki Ikura1, Nobuyuki Kaibuchi2, Masayuki Yamato2, Teruo Okano2, Yasuko Uchigata1 1 Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering Morito Sakuma1, Yoshikazu Kumashiro2, Masamichi Nakayama2, Nobuyuki Tanaka2, Yuji Haraguchi2, Kazuo Umemura1, Tatsuya Shimizu2, Masayuki Yamato2, Teruo Okano2 1Department of Physics, Tokyo University of Science, 2 Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.