3 articles published in JoVE
Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches Kazuhiko Shirakabe1,2, Masaaki Higashiyama1, Naoki Shibuya2, Kazuki Horiuchi1, Masayuki Saruta2, Ryota Hokari1 1Department of internal medicine, National Defense Medical College, 2Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.
Left Atrial Stenosis Induced Pulmonary Venous Arterialization and Group 2 Pulmonary Hypertension in Rat Ping Yu Xiong*1,2, Shunsuke Baba*3,4, Naritomo Nishioka3,5, Yoshitaka Fujimoto3, Stephen L. Archer1, Susumu Minamisawa1,4 1 Left atrial stenosis (LAS) is a novel surgical technique used for studying group 2 pulmonary hypertension (PH) and mechanisms underlying pulmonary venous arterialization. Here, we present a protocol to constrict the left atrium using a titanium clip to cause pulmonary venous arterialization and moderate PH in a rat.
A Surgical Procedure for the Administration of Drugs to the Inner Ear in a Non-Human Primate Common Marmoset (Callithrix jacchus) Sho Kurihara*1,2, Masato Fujioka*3, Tomohiko Yoshida*1,2, Makoto Koizumi4, Kaoru Ogawa3, Hiromi Kojima2, Hirotaka James Okano1 1Division of Regenerative Medicine, Jikei University School of Medicine, 2Department of Otorhinolaryngology, Jikei University School of Medicine, 3Department of Otorhinolaryngology, Head and Neck Surgery, Keio University School of Medicine, 4Laboratory Animal Facilities, Jikei University School of Medicine We report a surgical method to administrate drugs to the inner ear of a non-human primate, the common marmoset (Callithrix jacchus), via the round window membrane.