3 articles published in JoVE
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice Hirofumi Nishizono*1,2,3, Mohamed Darwish*4,5, Hideki Uosaki6,7, Nanami Masuyama8,9,10, Motoaki Seki8,11, Hiroyuki Abe3, Nozomu Yachie8,9,10,12,13, Ryohei Yasuda1 1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.
Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy Afrina Mustari*1, Izumi Nishidate*1, Md. Abdul Wares1,6, Takaaki Maeda2, Satoko Kawauchi3, Shunichi Sato3, Manabu Sato4, Yoshihisa Aizu5 1Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology, 2Department of Mechanical Engineering, Kushiro National College of Technology, 3Division of Bioinformation and Therapeutic Systems, National Defense Medical College Research Institute, 4Graduate School of Science and Engineering, Yamagata University, 5College of Design and Manufacturing Technology, Muroran Institute of Technology, 6Department of Livestock Services, Ministry of Fisheries and Livestock, Government of Bangladesh Here, we demonstrate how agarose-based tissue-mimicking optical phantoms are made and how their optical properties are determined using a conventional optical system with an integrating sphere.
Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging Izumi Nishidate1, Afrina Mustari1, Satoko Kawauchi2, Shunichi Sato2, Manabu Sato3 1Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology, 2Division of Biomedical Information Sciences, National Defense Medical College Research Institute, 3Graduate School of Science and Engineering, Yamagata University The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system.