Daiichi Sankyo Co., Ltd. 2 articles published in JoVE Biology Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR Takuma Iguchi1, Noriyo Niino1, Satoshi Tamai1, Ken Sakurai1, Kazuhiko Mori1 1Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd. This report describes a protocol for measuring the absolute levels of plasma miRNA, using quantitative real-time reverse transcription PCR with or without pre-amplification. This protocol affords better understanding of the quantity of plasma miRNAs and allows qualitative assessment of corresponding data from different studies or laboratories. Medicine A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis Venkatesh Mani1, Nadia Alie1, Sarayu Ramachandran1, Philip M. Robson1, Cecilia Besa1, Gregory Piazza2, Michele Mercuri3, Michael Grosso3, Bachir Taouli1, Samuel Z. Goldhaber2, Zahi A. Fayad1 1Department of Radiology, Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, 2 The goal of this study is to use magnetic resonance venography with long-circulating gadolinium-based contrast agent and direct thrombus imaging for quantitative evaluation of DVT volume in a multicenter, clinical trial setting. Inter- and intra-observer variability assessments were conducted, and reproducibility of the protocol was determined.
