2 articles published in JoVE
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva Li Xiao1, Hisashi Okamura2, Yasuo Kumazawa2 1Department of Pharmacology, The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and periodontitis.
A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side Stefanie Dinner1, Julia Borkowski1, Carolin Stump-Guthier1, Hiroshi Ishikawa2, Tobias Tenenbaum1, Horst Schroten1, Christian Schwerk1 1Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 2Department of NDU Life Sciences, Nippon Dental University The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.