Aichi Human Service Center

1 article published in JoVE

• Biology

  
  Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections Yuka Mizutani¹, Daisuke Kuga², Machiko Iida¹, Kaori Ushida³, Tsuyoshi Takagi¹, Yoshihito Tokita¹, Masahide Takahashi³, Masato Asai^{1,3 1}Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, ²Surgery Department, Anjo Kosei Hospital, ³Department of Pathology, Nagoya University Graduate School of Medicine Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.