3 articles published in JoVE
Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns Masami Yokogawa1, Tomoyo Kurebayashi2, Kazuki Soma3, Hiroichi Miaki1, Takao Nakagawa4 1Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Rehabilitation Section, Higashimatsuyama Municipal Hospital, 3Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 4Department of Physical and Rehabilitation Medicine, Kanazawa Medical University Here, we present a protocol to assess two deep breathing patterns of natural and diaphragmatic breathing for their effectiveness and ease of execution. Fifteen participants were selected, utilizing an electrocardiograph and expired gas analyzer for measurement of the ventilatory parameters, together with visual assessment by video capture of thoracoabdominal movement.
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice Munenori Ono1, Shinji Muramoto1, Lanlan Ma1, Nobuo Kato1 1Department of Physiology, Kanazawa Medical University This work introduces a method to perform an optogenetic single-unit recording reliably from an awake mouse using a custom-made glass optrode.
Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes Hidehito Saito1,2, Kumiko Iwabuchi3, Noemi Fusaki4,5, Fumito Ito3,6 1Department of Surgery, University of Michigan, 2Department of Biochemistry II, Kanazawa Medical University, 3Center for Immunotherapy, Roswell Park Cancer Institute, 4DNAVEC Corporation, 5Department of Ophthalmology, Keio University School of Medicine, 6Department of Surgical Oncology, Roswell Park Cancer Institute The goal of this protocol is to show the protocol for reprogramming melanoma tumor-infiltrating lymphocytes into induced pluripotent stem cells.