The University of Tokyo Hospital

5 articles published in JoVE

• Biology

  
  Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents Takahiro Maekawa*¹, Naoyoshi Sakitani*¹, Youngjae Ryu¹, Atsushi Takashima², Shuhei Murase¹, Julius Fink³, Motoshi Nagao¹, Toru Ogata⁴, Masahiro Shinohara¹, Yasuhiro Sawada^{5 1}Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities, ²Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities, ³Department of Metabolism and Endocrinology, Graduate School of Medicine, Juntendo University, ⁴Department of Rehabilitation Medicine, Graduate School of Medicine, The University of Tokyo, ⁵Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities The present protocol describes a custom-designed ''passive head motion'' system, which reproduces mechanical accelerations at rodents' heads generated during their treadmill running at moderate velocities. It allows dissecting mechanical factors/elements from the beneficial effects of physical exercise.

• Immunology and Infection

  
  Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes Naoyoshi Sakitani*¹, Takahiro Maekawa*¹, Kumiko Saitou^1,2, Katsuhiko Suzuki³, Shuhei Murase^1,4, Masakuni Tokunaga¹, Daisuke Yoshino⁵, Keisuke Sawada⁶, Atsushi Takashima⁷, Motoshi Nagao¹, Toru Ogata¹, Yasuhiro Sawada^{1,8 1}Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities, ²Graduate School of Sport Sciences, Waseda University, ³Faculty of Sport Sciences, Waseda University, ⁴Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Tokyo, ⁵Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, ⁶University of Cincinnati College of Medicine, ⁷Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities, ⁸Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities Here we describe the protocols for applying defined mechanical loads to mouse calves and for monitoring the concomitant intramuscular pressure changes. The experimental systems that we have developed can be useful for investigating the mechanism behind the beneficial effects of physical exercise and massage.

• Developmental Biology

  
  Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition Yu Mikami^1,2, Hirotaka Matsuzaki², Hideyuki Takeshima², Kosuke Makita², Yasuhiro Yamauchi², Takahide Nagase^{2 1}Department of Clinical Laboratory, The University of Tokyo Hospital, ²Department of Respiratory Medicine, The University of Tokyo Hospital Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

• Engineering

  
  Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis Markus Wilde¹, Satoshi Ohno¹, Shohei Ogura¹, Katsuyuki Fukutani¹, Hiroyuki Matsuzaki^{2 1}Institute of Industrial Science, The University of Tokyo, ²Micro Analysis Laboratory, Tandem accelerator, The University Museum, The University of Tokyo We illustrate the application of ¹H(¹⁵N,αγ)¹²C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.

• Biology

  
  Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy Takumi Higaki^{1 1}Graduate School of Frontier Sciences, The University of Tokyo The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.