3 articles published in JoVE
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition Yu Mikami1,2, Hirotaka Matsuzaki2, Hideyuki Takeshima2, Kosuke Makita2, Yasuhiro Yamauchi2, Takahide Nagase2 1Department of Clinical Laboratory, The University of Tokyo Hospital, 2Department of Respiratory Medicine, The University of Tokyo Hospital Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis Markus Wilde1, Satoshi Ohno1, Shohei Ogura1, Katsuyuki Fukutani1, Hiroyuki Matsuzaki2 1Institute of Industrial Science, The University of Tokyo, 2Micro Analysis Laboratory, Tandem accelerator, The University Museum, The University of Tokyo We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy Takumi Higaki1 1Graduate School of Frontier Sciences, The University of Tokyo The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.